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Definition - We can define to cell separation as  sorting or isolation of cells(homogeneous population) from a mixture or sample. Cell separation is a important tool witch used in clinical , biotechnology, and research work, many physical method are used today. in physical separation cell sorted on physical properties-

•     Cell density
•     Cell size
•     Affinity of antibodies on cell surface epitopes.
•     Light scatter or fluorescent emission by labeled cells.

Separation by centrifugal-cell separated by this method on basis of cell size and its sedimentation velocity. centrifugal elutriation the most advanced technique based on centrifugal force.an centrifuge used the sedimentation principle for work witch create to denser substances to sorted out along the radial direction.










Separation by fluorescence- activated cell sorting(FACS)- Is a powerful technique witch sorted to cells on basis of differences  that detected by a light scatter example cell size or by fluorescence emission example by penetrated RNA, DNA. the method include single cell stream passing through laser beam so that the light witch reflected by cells  detected by a detector  and recorded this data. two instruments are used for fluorescence activated cell sorting

• Florescence activated cell sorter

• Flow Cytometer.

PHYSICAL CELL SEPARATION

Application of cell separation- that mainly applied for clinical purpose and in biotechnology used for library screening or strain development. used in protein engineering and screening for protein overproduction,  for cell sorting that is true “what you screen for than you get it” that help in development and design of analytical methods.


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